Regulatory

Part:BBa_K758003:Design

Designed by: Takaaki Takeda   Group: iGEM12_KIT-Kyoto   (2012-09-17)


UAS, this part consists of five UAS sequences and hsp70 TATA.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR pUAST in order to amplify this sequence.


Source

Five UAS sequences, hsp70, and TATA

References